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Neurotransmitters are crucial for the proper functioning of neural systems, with dopamine playing a pivotal role in cognition, emotions, and motor control. Dysregulated dopamine levels are linked to various disorders, underscoring the need for accurate detection in research and diagnostics. Single-stranded DNA (ssDNA) aptamers are promising bioreceptors for dopamine detection due to their selectivity, improved stability, and synthesis feasibility. However, discrepancies in dopamine specificity have presented challenges. Here, we surface-functionalized a nano-plasmonic biosensing platform with a dopamine-specific ssDNA aptamer for selective detection. The biosensor, featuring narrowband hybrid plasmonic resonances, achieves high specificity through functionalization with aptamers and passivation processes. Sensitivity and selectivity for dopamine detection are demonstrated across a wide range of concentrations, including in diverse biological samples like protein solutions, cerebrospinal fluid, and whole blood. These results highlight the potential of plasmonic “aptasensors” for developing rapid and accurate diagnostic tools for disease monitoring, medical diagnostics, and targeted therapies. 

Rumble has emerged as a prominent platform hosting contro- versial figures facing restrictions on YouTube. Despite this, the academic community’s engagement with Rumble has been minimal. To help researchers address this gap, we intro- duce a comprehensive dataset of about 6.7K podcast videos from August 2020 to December 2022, amounting to over 5.6K hours of content. Besides covering metadata of these podcast videos, we provide speech-to-text transcriptions for future analysis. We also provide speaker diarization informa- tion, a collection of 168K unique representative images from podcast videos, and face embeddings of more than 400K ex- tracted faces. With the rise of the influence of podcasts and populist figures, this dataset provides a rich resource to iden- tify challenges in cyber social threats in a relatively underex- plored space. 

Monitoring chemical levels is crucial for safeguarding both the environment and public health. Elevated levels of ammonia, for instance, can harm both humans and aquatic ecosystems, often indicating contamination from agriculture, industry, or sewage. Developing portable, high-resolution, and affordable methods for in situ monitoring of ammonia is thus imperative. Plasmonic sensors offer a promising solution, detecting ammonia by correlating changes in their optical response to the target analyte’s concentration. While they are highly sensitive and can be fabricated in a variety of portable and user-friendly formats, some still require reagents or expensive optical equipment, which hinder their widespread adoption. Here, we present a self-assembled nanoplasmonic colorimetric sensor capable of directly detecting ammonia concentrations in aqueous matrices. The proposed sensor exploits the plasmonic resonance of the nanostructures to transduce changes in the chemical environment into alterations in color, offering a label-free method for real-time analysis. The sensor is fabricated using a self-assembling technique compatible with low-cost mass production based on aluminum and aluminum oxide, ensuring affordability and avoiding the use of other toxic chemicals. We developed a model to predict ammonia concentrations based on visible color change of the sensor, achieving a detection limit of 8.5 ppm. Furthermore, to address the need for on-site detection, we integrated smartphone technology for real-time color change analysis, eliminating the need for expensive, bulky optical instruments. Indeed, our approach offers a cost-effective, portable, and user-friendly solution for ammonia detection in water without the need for chemical reagents or spectrometers, making it ideal for field applications. Interestingly, this platform extends its applicability beyond ammonia detection, enabling the monitoring of various chemicals using a smartphone, without the need for any additional costly equipment. 

Online web communities often face bans for violating platform policies, encouraging their migration to alternative platforms. This migration, however, can result in increased toxicity and unforeseen consequences on the new platform. In recent years, researchers have collected data from many alternative platforms, indicating coordinated efforts leading to offline events, conspiracy movements, hate speech propagation, and harassment. Thus, it becomes crucial to characterize and understand these alternative platforms. To advance research in this direction, we collect and release a large-scale dataset from Scored -- an alternative Reddit platform that sheltered banned fringe communities, for example, c/TheDonald (a prominent right-wing community) and c/GreatAwakening (a conspiratorial community). Over four years, we collected approximately 57M posts from Scored, with at least 58 communities identified as migrating from Reddit and over 950 communities created since the platform's inception. Furthermore, we provide sentence embeddings of all posts in our dataset, generated through a state-of-the-art model, to further advance the field in characterizing the discussions within these communities. We aim to provide these resources to facilitate their investigations without the need for extensive data collection and processing efforts. 

Students’ beliefs about their abilities (called “lay theories”) affect their motivations, behaviors, and academic success. Lay theories include beliefs about the potential to improve intelligence (mindset), who (i.e., everyone or only some people) has the potential to be excellent in a field (universality), and whether reaching excellence in a field requires raw intellectual talent (brilliance). Research demonstrates that each of these beliefs influences students’ educational experiences and academic outcomes. However, it remains unclear whether they represent distinct latent constructs or are susceptible to the “jangle fallacy” (i.e., different names given to the same underlying construct). We conducted a multiphase, mixed-methods study to 1) evaluate whether mindset, universality, and brilliance beliefs represent conceptually and empirically discriminable concepts, and 2) evaluate whether mindset, universality, and brilliance beliefs contribute unique explanatory value for both psychosocial (e.g., sense of belonging) and academic outcomes (e.g., course grades). To address these questions, we developed and collected validity evidence for a new measure of science and math undergraduates’ lay theories, called the Undergraduate Lay Theories of Abilities (ULTrA) survey. Factor analyses suggest that mindset, brilliance, and universality are distinct and empirically discriminable constructs. Structural Equation Models indicate that each lay theory contributes unique predictive value to relevant outcomes. 

Objective: To assess whether co-culture with vitrified-warmed cumulus cells (CCs) in media drops improves rescue in vitro maturation (IVM) of previously vitrified immature oocytes. Previous studies have shown improved rescue IVM of fresh immature oocytes when cocultured with CCs in a three-dimensional matrix. However, the scheduling and workload of embryologists would benefit from a simpler IVM approach, particularly in the setting of time-sensitive oncofertility oocyte cryopreservation (OC) cases. Although the yield of developmentally competent mature metaphase II (MII) oocytes is increased when rescue IVM is performed before cryopreservation, it is unknown whether maturation of previously vitrified immature oocytes is improved after coculture with CCs in a simple system not involving a three-dimensional matrix. Design: Randomized controlled trial. Setting: Academic hospital. Patients: A total of 320 (160 germinal vesicles [GVs] and 160 metaphase I [MI]) immature oocytes and autologous CC clumps were vitrified from patients who were undergoing planned OC or intracytoplasmic sperm injection from July 2020 until September 2021. Interventions: On warming, the oocytes were randomized to culture in IVM media with CCs (+CC) or without CCs (-CC). Germinal vesicles and MI oocytes were cultured in 25 μL (SAGE IVM medium) for 32 hours and 20-22 hours, respectively. Main outcome measures: Oocytes with a polar body (MII) were randomized to confocal microscopy for analysis of spindle integrity and chromosomal alignment to assess nuclear maturity or to parthenogenetic activation to assess cytoplasmic maturity. Wilcoxon rank sum tests for continuous variables and the chi square or Fisher's exact test for categorical variables assessed statistical significance. Relative risks (RRs) and 95% confidence intervals (CIs) were calculated. Results: Patient demographic characteristics were similar for both the GV and MI groups after randomization to +CC vs. -CC. No statistically significant differences were observed between +CC vs. -CC groups regarding the percentage of MII from either GV (42.5% [34/80] vs. 52.5% [42/80]; RR 0.81; 95% CI: 0.57-1.15]) or MI (76.3% [61/80]; vs. 72.5% [58/80]; RR 1.05; 95% CI: 0.88-1.26]) oocytes. An increased percentage of GV-matured MIIs underwent parthenogenetic activation in the +CC group (92.3% [12/13] vs. 70.8% [17/24]), but the difference was not statistically significant (RR 1.30; 95% CI: 0.97-1.75), whereas the activation rate was identical for MI-matured oocytes (74.3% [26/35] vs. 75.0% [18/24], CC+ vs. CC-; RR 0.99; 95% CI: 0.74-1.32). No significant differences were observed between +CC vs. -CC groups for cleavage of parthenotes from GV-matured oocytes (91.7% [11/12] vs. 82.4% [14/17]) or blastulation (0 for both) or for MI-matured oocytes (cleavage: 80.8% [21/26] vs. 94.4% [17/18]; blastulation: 0 [0/26] vs. 16.7% [3/18]). Further, no significant differences were observed between +CC vs. -CC for GV-matured oocytes regarding incidence of bipolar spindles (38.9% [7/18] vs. 33.3% [5/15]) or aligned chromosomes (22.2% [4/18] vs. 0.0 [0/15]); or for MI-matured oocytes (bipolar spindle: 38.9% [7/18] vs. 42.9% [2/28]); aligned chromosomes (35.3% [6/17] vs. 24.1% [7/29]). Conclusions: Cumulus cell co-culture in this simple two-dimensional system does not improve rescue IVM of vitrified, warmed immature oocytes, at least by the markers assessed here. Further work is required to assess the efficacy of this system given its potential to provide flexibility in a busy, in vitro fertilization clinic.